Bullous Pemphigoid 180 and 230 Antibody, IgG
Order Name
B Pemph Ab
Test Number: 5194672
Revision Date 02/05/2024
Test Number: 5194672
Revision Date 02/05/2024
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
Bullous Pemphigoid 180 and 230 Antibody, IgG
|
Enzyme immunoassay (EIA) |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | 2 mL (1 mL) | Serum | Clot Activator (SST or Red No-Gel) | Frozen |
| Instructions | Specimen Type: Gel-Barrier Tube, Red-Top Tube, or Serum Transfer Tube Specimen Storage: FROZEN at -20 degrees C Specimen Collection: Serum samples may be stored for up to 8 hours at room temperature before freezing at -20 degrees C. Samples should not be repeatedly frozen and thawed. Specimen Stability: Ambient: Unstable, Refrigerated : Unstable, Frozen: 14 Days. (Freeze/thaw cycle: Stable x3) Cause for Rejection: Bacterial contamination, hemolysis, and lipemia. |
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| GENERAL INFORMATION | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Expected TAT | 7 days | |||||||||
| Clinical Use | This test is a preferred screening test for patients suspected to have bullous pemphigoid and its variants. The antibody levels can be used to monitor the effectiveness of drug treatment. The diverse spectrum of pemphigoid diseases is characterized by the formation of subepidermal blisters, which can develop in both the skin and mucous membranes. These conditions involve circulating autoantibodies that specifically target components found in the dermal-epidermal junction. Bullous pemphigoid (BP) presents with tense, swollen blisters that can appear on either inflamed or non-inflamed skin, with mucous membranes being rarely affected. The patient's serum contains IgG antibodies that primarily target hemidesmosomal proteins, namely BP180 and BP230. BP180 is a transmembrane glycoprotein with its primary immunogenic epitopes located in the extracellular 16th non-collagenous domain (BP180-NC16 A). Pemphigoid gestationis is a variant of BP that occurs in pregnant women and during the postpartum period, resulting in the development of urticarial plaques and/or tense blisters. Mucous membrane pemphigoid affects one or more mucous membranes such as the oral, ocular, genital and anal regions, and can also involve the skin. Patients with this condition exhibit low-titer IgG/IgA autoantibodies directed against components of the basement membrane zone, with BP180 and laminin 332 being the two primary targets. | |||||||||
| Performing Labcorp Test Code | 163995 | |||||||||
| Notes |
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| CPT Code(s) | 83516x2 | |||||||||
| Lab Section | Reference Lab | |||||||||