Euglobulin Clot Lysis Time
Order Name
EUG LYSIS
Test Number: 1500950
Revision Date 12/12/2022
Test Number: 1500950
Revision Date 12/12/2022
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
Euglobulin Clot Lysis Time
|
See Test Notes | 3244-1 |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | 2 mL (1 mL) | Plasma | Sodium Citrate 3.2% (Blue Top) | Frozen |
| Instructions | Specimen Type: Blue-top (sodium citrate) tube Specimen Storage: FREEZE Specimen Collection: CITRATED PLASMA SAMPLES SHOULD BE COLLECTED BY DOUBLE CENTRIFUGATION. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.[1] Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.[2,3] The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anti- coagulant with the blood. A discard tube is not required prior to collection of coagulation samples except when using a winged blood collection device (i.e. 'butterfly'), in which case a discard tube should be used.[4,5] When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue- top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentifuge for 10 minutes. Use a second plastic pipette to remove plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp 49482). FREEZE immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume. Special Instructions: Testing is performed at Esoterix Coagulation Laboratory: UY#300403 Specimen Stability: Ambient: Not Available, Refrigerated : Not Available, Frozen: Not Available |
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| GENERAL INFORMATION | |
|---|---|
| Expected TAT | 4 - 8 days |
| Performing Labcorp Test Code | 500055 |
| Notes | Methodology The euglobulin fraction of the plasma refers to plasma proteins that precipitate at low pH and decreased ionic strength.6 In the ELT test, plasma inhibitors of fibrinolysis are physically removed and the reaction of fibrinogen, plasminogen, and plasminogen activators are assayed. The precipitate also includes tissue plasminogen activators, plasminogen, plasmin, and fibrinogen. The inhibitors of lysis, α2-antiplasmin, and α2-macroglobulin, do not precipitate. In the test system, the euglobulin precipitate is redissolved in buffer, and clotting is initiated with calcium. The assay is performed in a microtiter plate. The time required for the intrinsic plasmin to lyse the fibrin clot equates to the euglobulin lysis time. Labcorp Test Code: 500055 |
| CPT Code(s) | 85360 |
| Lab Section | Reference Lab |