Methodologies


AbbreviationNameDescription
RIARadioimmunoassay (RIA) uses fixed-dose, low-level, radioactive-isotope- labeled antigen ("tracer") to compete with unlabeled antigen from the patient specimen for a fixed number of antibody binding sites. Traditional RIA is done with specific antibodies in liquid solution. Solid-phase RIA involves the use of antibody bound to solid support (e.g., tubes, glass beads or plastic fins). The amount of antigen in the specimen is determined by comparing the bound radioactivity with a standard curve.
 
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