Abbreviation | Name | Description |
AA | Atomic Absorption | Atomic Absorption |
AA-HPLC | Atomic Absorbtion, HPLC | Atomic Absorbtion, HPLC |
AAS | Atomic Absorption Spectrophotometry | Atomic Absorption Spectrophotometry |
Ab/Neutral | Antibody Neutralization | Antibody Neutralization |
ABIF | Avidin-Biotin Immunofluorescence | (ABIF) holds promise for more sensitive and specific amplification of indirect fluorescent antibody procedures. Antibody to the patient's specific antibodies is labeled with biotin, a compound capable of specifically binding avidin in high concentrations. Fluorescently labeled avidin is then added and fluorescent microscopy is used to detect the presence of the complexes. |
ACIF | Anticomplement Immunofluorescence | (ACIF) is a technique used to make certain indirect fluorescent antibody techniques more specific and sensitive. Here the fluorescent dye is conjugated to antibody directed at complement and then added to a complement-fixing complex of antigen and patient antibody. |
AE | Agarose Electrophoresis (AE) | Agarose Electrophoresis (AE) |
AEDS | Agarose Electrophoresis, Densitometry, Spectrophotometry | Agarose Electrophoresis, Densitometry, Spectrophotometry |
AGG | Aggregation | Platelet Aggregation |
AGHEP | Alkaline Gel Hemoglobin Electrophoresis | (AGHEP) is a technique for separation of ionic molecules (principally proteins) by the differential migration through a alkaline gel according to the size and ionic charge of the molecules in an electrical field. Smaller molecules with a more negative charge will travel faster and further through the alkaline gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualized by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain. |
Amine | Amine | Amine |
AnshLite | AnshLite(TM) Enzyme Linked Immunoassay | AnshLite(TM) Enzyme Linked Immunoassay |
Arsenazo | Arsenazo | Arsenazo |
AS | Atomic Spectroscopy | Atomic Spectroscopy |
AS-PCR | Allele-specific polymerase chain reaction (PCR) | Allele-specific polymerase chain reaction (PCR) |
ASA | Allele Specific Amplification | Allele Specific Amplification |
Assay Dep | Assay Dependant | Assay Dependant |
Atherotech | Atherotech | Atherotech |
Abbreviation | Name | Description |
Calc | Calculation | Calculation |
CE | Capillary Electrophoresis | Capillary Electrophoresis |
CEDIA | CEDIA | Cloned Enzyme Donor Immunoassay - The CEDIA method offers superior sensitivity and the best lot-to-lot consistency of any automated method. In addition, CEDIA assays are easier and less time-consuming than high-pressure liquid chromatography (HPLC) assays. |
CELLSEARCH | Veridex CellSearch | Veridex CellSearch |
CF | Complement fixation (CF) | (CF) is an exacting, complex yet sensitive procedure that detects the presence of a specific antigen-antibody reaction by causing the in vitro activation of complement via the classical pathway. If complement is not fixed, lysis of the pre-antibody-coated reagent erythrocytes occurs. Again, crude quantitation of antibodies is possible by determining the highest dilution (titer) at which lysis does not occur. The differentiation of specific antibody isotype is not possible. |
CF/ID/ELIS | Complement Fixation/Immunodiffusion/ELISA | Complement Fixation/Immunodiffusion/ELISA |
Chrom | Chromogenic | Chromogenic |
Chromatogr | Chromatography/spectrophotometry | Chromatography/spectrophotometry |
CIA | Chemiluminescence Assay | (CIA), including a subcategory using bioluminescence (biologically derived chemiluminescence agents), use the generation of light from oxidative chemical reactions as an indicator of the quantity of unbound luminescently labeled antigen. This allows quantitation of unlabeled antigen from patient specimens in a variety of homogeneous (single phase) or heterogeneous (multiple phase) immunoassay techniques. |
CIE | Counterimmunoelectrophoresis | (CIE) is a procedure in which oppositely charged antigen and antibody are propelled toward each other by an electrical field. This reduces the time necessary for visualization of the antigen-antibody reaction from 18-24 hours in ID to less than one hour and also substantially increases the sensitivity of the analysis. CIE has the capability of detecting concentrations of antigen/antibody 10 times smaller than the lowest concentrations measurable by DD or ID. |
CL/IA | Chemiluminescence, Immunoassay | Chemiluminescence, Immunoassay |
CLIA | Antibody Capture Chemiluminescence Immunoassay (CLIA) | Antibody Capture Chemiluminescence Immunoassay (CLIA) |
CLOT | Clot Detection | Clot Detection |
CMIA | Chemiluminescent Micropartical Immunoassay | Chemiluminescent Micropartical Immunoassay |
CNPG3 | CNPG3 | Reagent utilises 2-chloro-pnitrophenyl- |
CoA | Coagglutination | (CoA) is similar to the LA technique for detecting antigen (described above). Protein A, a uniformly distributed cell wall component of Staphylococcus aureus, is able to bind to the Fc region of most IgG isotype antibodies leaving the Fab region free to interact with antigens present in the applied specimens. The visible agglutination of the S. aureus particles indicates the antigen-antibody reaction. |
COLO | Colorimetric | Colorimetric |
Colo/Port | Colorimetric with Porter-Silber Reaction | Colorimetric with Porter-Silber Reaction |
Colo/Zim | Colorimetric with Modified Zimmerman Reaction | Colorimetric with Modified Zimmerman Reaction |
Comment | Comment | Comment |
Cop-Sulf | Copper Sulfate | Copper Sulfate |
COUL | Coulometric | The iontophoresis Sweat Test, a collection is elution of sweat in a plastic coil then analyzed by the coulometric titration of chloride with silver by a digital chloridometer. |
CQQCIA | Cell Culture/Quantitative CIA/ Semi-Quantitative CIA | Cell Culture/Quantitative Chemiluminescent Immunoassay/ Semi-Quantitative Chemiluminescent Immunoassay |
Cul/DFA | Culture / DFA | Microbiological Culture and DFA (DFA) is a general term for techniques which use the agglutination (macroscopic clumping) of particulate reagents as an indicator of the presence of an antigen-antibody reaction. Examples (HA, LA and CoA) follow. |
Cul/Stim | Cul/Stim | Ex-Vivo cell culture and Histamine analysis for Chronic Urticaria (CU) index. |
Cult | Culture | Microbiological Culture |
Cult/CIA | Cell Culture/Chemiluminescence | Cell Culture/Chemiluminescence |
Cyt-St | Cytochemical Stain | Cytochemical Stain |
Abbreviation | Name | Description |
E-RBC | Enzymatic-RBC Hemolysate | Enzymatic-RBC Hemolysate |
E/LCTMS | Enzymatic/Quantitative Liquid Chromatography-Tandem Mass Spectrometry | Enzymatic/Quantitative Liquid Chromatography-Tandem Mass Spectrometry |
ECIA | Electrochemiluminescent Immunoassay | Electrochemiluminescent Immunoassay |
ECL | Electrochemiluminescence (ECL) | Electrochemiluminescence (ECL) |
ECLIA | Electrochemiluminescence Immunoassay (ECLIA) | Electrochemiluminescence Immunoassay (ECLIA) |
ECRA | Extraction, Chromatography, Radioreceptor Assay | Extraction, Chromatography, Radioreceptor Assay |
EIA | Enzyme Immunoassay | (EIA) is the general term for an expanding technical arsenal of testing which allows a full range of quantitative analyses for both antigen and antibodies. These tests use color-changed products of enzyme-substrate interaction (or inhibition) to measure the antigen-antibody reaction. Examples of EIA procedures (EMIT, ELISA, MAC, MEIA) follow. |
EIA Ag Cap | EIA (Antigen Capture) | EIA (Antigen Capture) |
EIA/LCTMS | Qual EIA/Quant LC-TMS | Qualitative Enzyme Immunoassay/Quantitative Liquid Chromatography-Tandem Mass Spectrometry |
Elect-Chem | Electro Chemical | Electrochemical reaction with lead detection sensor |
ELISA | Enzyme-Linked Immunosorbent Assay (ELISA) | (ELISA) is a sensitive, heterogenous (multiple phase) analytical technique for quantitation of antigen or antibody in which enzyme-labeled antibody or antigen is bound to a solid support (e.g., tubes, beads, microtiter plate wells, plastic tines or fins). After addition of patient specimen and substrate, antigen, antibody or complex are detected by a color change indicating the presence of the product of an enzyme-substrate reaction. Direct ELISA is a technique for measuring antigen using competition for antibody binding sites between enzyme- labeled antigen and patient antigen. Indirect ELISA, or enzyme immunometric assay, measures antibody concentrations using bound antigen to interact with specimen antibodies. Enzyme-labeled reagent antibodies can be isotype-specific (i.e., capable of determining the presence of IgG, IgA, IgM or IgE classes which react with the antigen of interest). The specificity of indirect ELISA assays for IgM isotypes in some infectious diseases is limited by false-positive results due to IgM rheumatoid factor in the presence of IgG-specific antibodies. |
ELISA/ACIF | ELISA/ACIF/IFA | ELISA/ACIF/IFA |
ELISA/CLIA | Semi-Quantitative ELISA/Semi-Quantitative CLIA | Semi-Quantitative Enzyme-Linked Immunosorbent Assay(ELISA)// Semi-Quantitative Chemiluminescent Immunoassay(CLIA) |
ELISA/IFA | Enzyme-Linked Immunosorbent Assay/IFA | Enzyme-Linked Immunosorbent Assay/Indirect Fluorescent Antibody |
ELISA/LCMS | Qualitative ELISA/Quantitative LC-TMS | Qualitative Enzyme-Linked Immunosorbent Assay/Quantitative Liquid Chromatography-Tandem Mass Spectrometry |
ELISA/Neph | Enzyme Linked Immunosorbent Assay/Nephelometry | Enzyme Linked Immunosorbent Assay/Nephelometry |
ELISACFIFA | ELISA, CompFix, IFA | ELISA, CompFix, IFA |
ELVIS® | Enzyme-linked virus-inducible system (ELVIS®) | Enzyme-linked virus-inducible system (ELVIS®). ELVIS® HSV was developed using a transgenic baby hamster kidney-21 cell line. In this cell line, HSV infection causes the expression of a beta-galactosidase “reporter gene”, and it is the expression of this gene that can be used to monitor infection by HSV. Cultures positive by ELVIS® are confirmed by immunofluorescent staining. |
EMCD | Electromagnetic Mechanical Clot Detection | Electromagnetic Mechanical Clot Detection |
EMIT | Enzyme Multiplied Immunoassay Technique | (EMIT) is a homogeneous (single phase) EIA procedure in which the antigen being measured competes for a limited number of antibody binding sites with enzyme labeled antigen. The reagent antibody has the ability to block enzymatic activity when bound with the reagent enzyme-antigen complex preventing it's formation of product in the presence of substrate. The free antigen- enzyme complexes resulting from competition with measured antigen in the sample forms color-change products proportional to the concentration of antigen present in the specimen. |
Enz | Enzymatic | Enzymatic |
ENZ-Color | Enzymatic - Colorimetric | Enzymatic - Colorimetric |
ENZ-Spec | Enzymatic - Spectrophotometric | Enzymatic - Spectrophotometric |
EP | Electrophoresis | (EP) is a technique for separation of ionic molecules (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. Smaller molecules with a more negative charge will travel faster and further through the gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualized by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain. |
Eq-Dial | Equilibrium Dialysis | Equilibrium Dialysis |
ERD | Electronic Resistance Detection | Electronic Resistance Detection |
Ex-HP-HPLC | Extraction, High Pressure HPLC | Extraction, High Pressure HPLC |
Ex-RIA | Extraction - RIA | Extraction Radioimmunoassay (RIA) |
Ex-Vivo | Ex-Vivo challenge and cell culture/Histamine analysis | Ex-Vivo challenge and cell culture/Histamine analysis |
EZA | Enzymatic Assay (EZA) | Enzymatic Assay (EZA) |
Abbreviation | Name | Description |
FA | Fluorescent Antibody | (FA) assay is a general term for procedures which utilize the visual detection of fluorescent dyes coupled (conjugated) to antibodies which react with the antigen when present using fluorescent microscopy. FA allows a competent technologist to identify visually the site of the antigen-antibody reaction thereby rendering significant specificity. Variations are further explained below (DFA, IFA, ACIF, ABIF and Micro-IF). |
FC | Flow cytometry | (FC) is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerized instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorized first by size and then computer analyzed to sort the mixture of cellular elements into cell type by size. In addition, monoclonal antibodies to specific cell surface markers are conjugated to fluorescent dyes and each cell displaying appropriate fluorescent light emission is counted. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilized, even when the size of the cell is identical to other subset species. |
FCI | Flow Cytometric Immunophenotyping | |
FEIA | Enzyme immunoassay (EIA) | Enzyme immunoassay (EIA). A standard curve is used to calculate the specific IgG concentrations. The calibrators are referenced to the International Reference Preparation for serum immunoglobulins. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration. |
Ferene | Ferene | Ferene |
FIA | Fluoroimmunoassay | Fluoroimmunoassay, Luminex® Multiplex, assay using beads coupled with capsular polysaccharide antigens from Streptococcus pneumonia. The assay is calibrated against U.S. Food and Drug Administration reference serum 89SF. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration. |
FISH | Fluorescence in Situ Hybridization | (FISH) is part of the domain of cytogenetics and has very specific applications. FISH allows for detection of specific numerical and structural chromosome abnormalities. The method allows for screening of a larger number of cells (>200 or more) for analysis than classical chromosomal cytogenetics. |
Fl | Fluorometry | Fluorometry |
Floc | Flocculation | Flocculation |
FMI | FMI | Fluorescent Microsphere Immunoassay |
Formalin c | Formalin concentrate and trichrome stain | |
FPD | Freezing Point Depression | Freezing Point Depression |
FPIA | Fluorescence Polarization Immunoassay | (FPIA) is a technique which takes advantage of the increased polarization (non-random propagation of emission) of fluorescent light emissions when a fluorescently labeled antigen is bound by reagent antibody. The higher the concentration of unlabeled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarization of the fluorescent light emission. Standard calibration yields quantitative results. |
FR-Neph | Fixed Rate Nephelometry | Fixed Rate Nephelometry |
Frz-Point | Freezing Point | Freezing Point |
FT-Neph | Fixed Time Nephelometry | Fixed Time Nephelometry |
Abbreviation | Name | Description |
H | Hematofluorometry (H) | Hematofluorometry (H) |
HA | Hemagglutination | (HA) is a technique for detecting specific antibodies which, when present, will cause antigen-coated reagent erythrocytes to agglutinate. Crude quantitation of the antibodies can be achieved by performing a serial dilution of the patient serum and noting the highest dilution (titer) at which agglutination is still present. |
Han Stain | Hansel stain | a stain used to detect eosinophils in urine or other body fluids, the eosinophils staining red against a background of blue. |
HCII | Hybrid Capture II | Hybrid Capture II |
Hemolysis | Hemolysis | Hemolysis |
Hemox | Hemoximeter | Hemoximeter |
Hexokinase | Hexokinase | Hexokinase |
HI | Hemagglutination Inhibition | (HI), also abbreviated HAI, is a variation of the HA technique. Some viral antigens, when coated on erythrocytes, spontaneously cause agglutination in the absence of antibody. In these situations, the specific antigen-antibody reaction actually prevents the agglutination of reagent RBCs. HAI cannot differentiate between isotypes of specific antibodies (IgG, IgA or IgM) although positive HAI analysis of specimens treated with Staphylococcus aureus Protein A (discussed above under CoA) to remove the IgG isotype antibodies has been used to imply the presence of specific IgM antibodies to the specific viral antigen. The crude quantitation of the specific antibodies is possible using serial dilution (titer). |
HPLC | High Performance Liquid Chromatography | High Performance Liquid Chromatography |
HPLC | High-pressure liquid chromatography (HPLC) | High-pressure liquid chromatography (HPLC) |
HPLC FLD | High-pressure liquid chromatography (HPLC) with fluorometric detection | High-pressure liquid chromatography (HPLC) with fluorometric detection |
HPLC TM | High-pressure liquid chromatography (HPLC) tandem mass spectrometry. | High-pressure liquid chromatography (HPLC) tandem mass spectrometry. |
HPLC/IEC/S | HPLC/Ion Exchange Chromatography/Quantitative Spectrophotometry | HPLC/Ion Exchange Chromatography/Quantitative Spectrophotometry |
HPLC/LCMS | HPLC/LCMSMS | HPLC/LCMSMS |
HPLC/TMS | HPLC/Tandem Mass Spectrometry | HPLC/Tandem Mass Spectrometry |
HPLC/UV | High-pressure liquid chromatography with ultraviolet detection (HPLC/UV) | |
Abbreviation | Name | Description |
I-PCR/El | Inverse Polymerase Chain Reaction/Electrophoresis | Inverse Polymerase Chain Reaction/Electrophoresis |
IA | Immunoassay (IA) | Immunoassay (IA) |
IA/IB | Immunofluorescence, Immunoblot | Immunofluorescence, Immunoblot |
IA/KAP | Immunoassay / Kinetic Alkaline Picrate (IA/KAP) | Immunoassay / Kinetic Alkaline Picrate (IA/KAP) |
IA/LCMSMS | Immunoassay; Liquid Chromatography/Tandem Mass Spectrometry | Immunoassay; Liquid Chromatography/Tandem Mass Spectrometry |
IC | ImmunoCard | Immunochromatographic rapid test utilizing monoclonal antibodies labeled with red-colored gold particles. |
ICA | Immunocytochemical Assay | (ICA) involves the computerized assessment of microscopic fields following DFA, IFA or indirect or direct IP analysis of biopsy tissue from the patient. In addition to improved specificity with the removal of operator subjectivity, the quantifiability of results through computer data analysis of color, intensity and concentration has only begun to be realized. |
ICAP | ImmunoCAP | ImmunoCAP Allergy testing |
ICFA | ImmuKnow Cell Function Assay | ImmuKnow Cell Function Assay |
ICMA | Immunochemiluminometric (ICMA) | Immunochemiluminometric (ICMA) |
ICP/MS | Inductively-Coupled Plasma/Mass Spectrometry | Inductively-Coupled Plasma/Mass Spectrometry |
ICP/MS AS | Inductively Coupled Plasma/Mass Spectrometry (ICP/MS) or Atomic Spectroscopy (AS) | Inductively Coupled Plasma/Mass Spectrometry (ICP/MS) or Atomic Spectroscopy (AS) |
ICP/OES | Inductively-Coupled Plasma/Optical Emission Spectroscopy | Inductively-Coupled Plasma/Optical Emission Spectroscopy |
ID | Immunodiffusion | (ID), also called Double diffusion (DD) or the Ouchterlony technique, is the classical procedure used to detect the presence of antibodies and determine their specificity by visualization of "lines of identity" (precipitin lines). These precipitin lines (precipitated antigen-antibody complexes) form where the binding concentrations of antigen and antibody are equivalent. Patient serum diffuses from one well through the gel and reacts with a known specific antigen (or antibody) which diffuses through the gel from a second well. DD is strictly qualitative, although the density of the precipitin line and the distance of the line from the sample well may give some indication of the antibody concentration. |
IEP | Immunoelectrophoresis | (IEP) is a two-step procedure which first involves the electrophoretic separation of proteins, followed by the linear diffusion of antibodies into the electrophoretic gel from a trough which extends through the length of the gel adjacent to the electrophoretic path. The antigen-antibody reactions produce precipitin arcs at positions where equivalence occurs. Although quantitation is subjective, an experienced eye can determine not only the presence of the antigen but, through visual comparison to normal control sera, may discriminate relative increases or decreases of antigen by gauging the length and density of the precipitin arcs at positions established for specific antigens using known standards. |
IFA | Indirect Fluorescent Antibody | (IFA) is the detection of antibodies to specific antigenic material in the substrate using fluorescent microscopy. Using fluorescently conjugated antibodies which are specific for a particular isotype of antibody, it is possible to distinguish IgG, IgA and IgM isotypes of specific antibodies using IFA. This sensitive technique is highly specific in well-trained hands and recent developments in the establishment of internationally recognized standard materials have led to accurate quantitation of antibody concentrations through endpoint titration (the highest serial dilution of specimen at which specific fluorescence remains) and through measuring visual intensity of fluorescence compared to known reference standard material. |
IFA | Immunofluorescence Assay (IFA) | Immunofluorescence Assay (IFA) |
IFCC NADH | IFCC;UV/NADH | IFCC;UV/NADH |
IFE-EP | Immunofixation Electrophoresis | Immunofixation Electrophoresis |
IFF | Indirect immunofluorescence (IFF) | Indirect immunofluorescence (IFF) |
IFIX | Immunofixation | (IFIX) is a powerful enhancement of immunoelectrophoresis in which a series of post-electrophoretic gel slabs are layered with cellulose-acetate gels saturated with specific antibodies. The resulting antigen-antibody complexes fixed on the second gel may then be stained, allowing sensitive and specific qualitative visual identification of paraproteins by electrophoretic position. |
iFOBT | Immunochemical Fecal Occult Blood Test | Hemoccult® ICT is the latest and most advanced fecal occult blood test (FOBT) in the Hemoccult product line. An immunochemical FOBT (iFOBT) with high specificity and sensitivity, Hemoccult ICT is clinically proven to detect bleeding associated with more cancers and polyps than traditional guaiac-based FOBTs. Because of its superior specificity for lower GI bleeding, Hemoccult ICT is an ideal screening tool for colorectal cancer (CRC), and this specificity also reduces the number of false positive test results. Safe and non-invasive, Hemoccult ICT is very patient friendly, as there are no diet or drug restrictions for patients to follow while they are using it, which helps increase patient compliance. |
IHC | Immunohistochemistry | Immunohistochemistry |
ImmDOT | ImmunoDOT | ImmunoDOT |
IMMENZ | Immunoenzymatic | Immunoenzymatic |
IMMP | Immunoprecipitin | Immunoprecipitin |
immQT | Immunologic, quantitative | Immunologic, quantitative |
IMMTb | Immunoturbidimetry | Immunoturbidimetry |
IMNeph | Immunonephelometry | Immunonephelometry |
IMOB | Ion Mobility | Ion Mobility |
InBC | Insulin-I125 binding capacity | Insulin-I125 binding capacity |
Infrared S | Infrared Spectroscopy | |
Int/Use | Internal Use Test | Internal Use Test |
INTERP | Interpretive information. | Interpretive information. |
INV | Signal Amplification, Invader chemistry detection | The Invader® platform is a proprietary technique by Third Wave Molecular Diagnostics. |
IP | Immunoperoxidase | (IP) assays are analogous to IFA in that antibody presence is identified on antigenic substrates visually. However, in the indirect IP instead of fluorescent dye-antibody conjugates, enzyme-antibody conjugates (principally peroxidase enzymes) are reacted with their corresponding substrates to produce a product which can be seen with a light microscope, eliminating the requirement for costly fluorescent microscopic equipment. |
IRMA | Immunoradiometric Assay | (IRMA) uses low-level radioactively labeled specific antibody to quantitate low concentration compounds. In IRMA, a first antibody is presented on solid-phase (coated on tubes or beads). After binding the antigen present in the sample, a second radioactively labeled antibody is added. Radioactivity remaining after washing the solid phase is proportional to the concentration of antigen present in the sample and is quantitated by comparison to a standard curve. |
ISE | Ion-Selective Electrode | Ion-Selective Electrode |
ISO-F | Isolectric Focusing | Isolectric Focusing |
Abbreviation | Name | Description |
L-FR NEPH | L-FR NEPH | Latex, Fixed Rate Time Nephelometry |
L/P(NAD) | Lactate - Pyruvate (NAD) | Lactate - Pyruvate (NAD) |
LA | Latex agglutination | (LA), also known as latex particle agglutination, for detection of antibodies is identical to HA in principle, but the substitution of smaller, antigen-coated latex particles for erythrocytes results in improved sensitivity and reagent longevity. Alternatively, antibodies can be absorbed to the latex particles (under appropriate ionic and pH conditions) by binding to the Fc region of antibodies, leaving the Fab region free to interact with antigens present in the applied specimens. This phenomenon has made LA a popular technique for detecting antigens as well. |
LAMP | LAMP | Is a molecular method of Loop mediated isothermal Amplification called Illumigene. Illumigene, by Meridian Bioscience, is an FDA approved technology providing better sensitivity over older EIA methodology. |
LC/MS | Liquid Chromatography/Mass Spectroscopy | Liquid Chromatography/Mass Spectroscopy |
LC/MS FD | Liquid chromatography/tandem mass spectrometry (LC/MS-MS) fluorescence detector | Liquid chromatography/tandem mass spectrometry (LC/MS-MS) fluorescence detector |
LC/MS/ED | Liquid Chromatography Mass Spectrometry, Equilibrium Dialysis | Liquid Chromatography Mass Spectrometry, Equilibrium Dialysis |
LC/MS/MS | Liquid Chromatography/Tandem Mass Spectrometry | Liquid Chromatography/Tandem Mass Spectrometry (LC/MS-MS) is a technique combining the principles of chromatography and mass spectrometry. Chromatographic separation of the analytes is based on the difference of their distribution between a mobile liquid phase and a stationary phase. Mass spectrometry measures ions based on the mass to charge m/z ratio. In LCMS-MS, following a liquid chromatography separation, the sample is introduced into a mass spectrometer, where the molecules are ionized, fragmented, and the fragments are sorted based on their m/z ratio, then subsequently introduced to a second mass spectrometer for further fragmentation, sorting and quantitation. This technique is highly specific and sensitive. |
Leumeta | Leumeta(TM) | Leumeta(TM) |
LIA | Latex Immunoassay Agglutination | (LIA)-When a monochromatic beam of light is passed through a suspension of microlates particles which have specific antibodies attached, the light is only slightly absorbed. If the specific antigen is added, the latex particles agglutinate causing the amount of light absorbed to increase. This increase in light absorption is proportional to the amount of antigen present. |
LiBaSys | Liquid-phase binding assay (LiBaSys) | Liquid-phase binding assay (LiBaSys) |
Line Blot | Line Blot | Line Blot |
LIPA | LIPA | Line probe assay (LiPA) |
LPBAS | Liquid-phase Binding Assay System | Liquid-phase Binding Assay System |
Abbreviation | Name | Description |
MAC ELISA | IgM Antibody Capture ELISA | (MAC ELISA) has been developed to impart significant improvement in assay specificity to indirect ELISA procedures for IgM isotype antibodies. Solid-phase support (usually microtiter plate wells) are coated with anti-human IgM antibodies capable of binding all IgM isotype antibodies present in the specimen. Reagent antigen is then added, followed by enzyme-labeled antigen- specific antibodies. If IgM antibodies specific for the antigen in question are present, the "sandwich" complex will result in enzymatic color-change proportional to the concentration of IgM-specific antibody present. This technique appears to be the method of choice in many highly specific and more sensitive assays for IgM infectious disease antibodies. |
Macr/Micr | Macroscopic and Microscopic examination | Macroscopic and Microscopic examination |
MAFD | Multi-Analyte Fluorescent Detection | Multi-Analyte Fluorescent Detection |
MAID | MAID | (Multi-Analyte Immunodetection) |
MC | Microscopy | (MC) use of a manually operated microscope. |
MEI | Microparticle Enzyme Immunoassay | Microparticle Enzyme Immunoassay |
MEIA | Microparticle enzyme immunoassay | (MEIA) is a technique in which the solid-phase support consists of very small microparticles in liquid suspension. Specific reagent antibodies are covalently bound to the microparticles. Antigen, if present, is then "sandwiched" between bound antibodies and antigen-specific, enzyme- labeled antibodies. Antigen-antibody complexes are detected and quantitated by analysis of fluorescence from the enzyme-substrate interaction. |
MFI | Multiplex flow immunoassay | Multiplex flow immunoassay |
MICF/ELISA | Microfluidics ELISA | Microfluidics ELISA |
miRNA | MicroRNA (miRNA) Profiling | MicroRNA (miRNA) Profiling |
Miro-IF | Micro-immunofluorescence | (Micro-IF) is really multiple IFA. Several different substrates are arranged in specific locations on a single microscope slide well allowing a rapid, simultaneous IFA on each substrate. |
Mixed Tech | Mixed Technology | Mixed Technology |
MLDPA | Multiplex Ligation-dependent Probe Amplification | Multiplex Ligation-dependent Probe Amplification |
MLP | Micro Latex Particle | Micro Latex Particle - Mediated Immunoassay |
Mod Jaffe | Modified Jaffe, Kinetic | a fixed time reaction where the picrate is added and the rate of the reaction is measured at a specific wavelength to determine the amount of creatinine in the sample. |
Mol-HLA | Molecular HLA | Molecular HLA oligotyping is determined utilizing PCR and sequence specific oligonucleotide probes, unless otherwise indicated. |
MP-MIA | Microlatex Particle-Mediated Immunoassay | Microlatex Particle-Mediated Immunoassay |
MPS | Massively Parallel Sequencing | Massively Parallel Sequencing |
MPS/CGHM | Massively Parallel Sequencing/Exonic Oligonucleotide-based CGH Microarry | Massively Parallel Sequencing/Exonic Oligonucleotide-based CGH Microarry |
Ms | Mass Spectrometry (MS) | Mass Spectrometry (MS) |
Multiplex | Multiplex PCR | Multiplex PCR |
Abbreviation | Name | Description |
PA | Particle Agglutination | Particle Agglutination |
Path Rep | Pathology Report | Pathology Report |
PatInfo | Patient Information | Patient Information |
PCR | Polymerase Chain Reaction | (PCR) is a highly efficient method to amplify low levels of specific DNA sequences in a sample to reach the threshold of detection. Two short DNA "primers", oligonucleotides (small portions of a single DNA strand) specific for the pathogenic DNA sought whose sequence flanks that section of DNA to be amplified, are used. Repeated cycles of DNA denaturation (separation of the double DNA strands), primer annealing (recombination of the double-stranded structure) and extension of the primed DNA sequence (by the enzyme DNA polymerase in the presence of added purine and pyrimidine bases) are performed. Each cycle doubles the amount of specific DNA sequence present and results in an exponential accumulation of the DNA fragment being amplified. The reaction products are hybridized to a radioactively labeled DNA segment complementary to a short sequence of the amplified DNA. Following electrophoresis, the radiolabeled product of specific size is detected by autoradiography. |
PCR-SSO | PCR-SSO | Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes (SSO) |
PCR/CE | PCR/Capillary Electrophoresis | Polymerase Chain Reaction/Capillary Electrophoresis |
PCR/CE | Polymerase Chain Reaction/Capillary Electrophoresis | Polymerase Chain Reaction/Capillary Electrophoresis |
PCR/DNA/ED | PCR/DNA Hybridization/Electrochemical Detection | PCR/DNA Hybridization/Electrochemical Detection |
PCR/FA | Polymerase Chain Reaction/Fragment Analysis | Polymerase Chain Reaction/Fragment Analysis |
PCR/FA | Polymerase Chain Reaction/Fragment Analysis | Polymerase Chain Reaction/Fragment Analysis |
PCR/FM | Polymerase Chain Reaction/Fluorescence Monitoring | Polymerase Chain Reaction/Fluorescence Monitoring |
PCR/FM | PCR/Fluorescence Monitoring | Polymerase Chain Reaction/Fluorescence Monitoring |
PCR/FRET | Real Time PCR/Fluorescence Resonance Energy Transfer | Real Time Polymerase Chain Reaction/Fluorescence Resonance Energy Transfer |
PCR/INV | PCR and Invader Plus® detection | PCR and Invader Plus® detection |
PCR/MPS | PCR/Massively Parallel Sequencing | Polymerase Chain Reaction/Massively Parallel Sequencing |
PCR/NE/FA | PCR/Single Nucleotide Extensions/Fragment Analysis | PCR/Single Nucleotide Extensions/Fragment Analysis |
PCR/PS | Polymerase Chain Reaction/Pyrosequencing | Polymerase Chain Reaction/Pyrosequencing |
PCR/RT | Polymerase Chain Reaction/Real Time | Polymerase Chain Reaction/Real Time |
PCR/S | Polymerase Chain Reaction/Sequencing | Polymerase Chain Reaction/Sequencing |
PCR/S/MLPA | PCR/Sequencing/Multiplex Ligation-dependent Probe Amp | Polymerase Chain Reaction/Sequencing/Multiplex Ligation-dependent Probe Amplification |
PCR/SNPE | Polymerase Chain Reaction/Single Nucleotide Primer Extension | Polymerase Chain Reaction/Single Nucleotide Primer Extension |
PCR/SNPE | Polymerase Chain Reaction (PCR) / Single Nucleotide Primer Extension | Polymerase Chain Reaction (PCR) / Single Nucleotide Primer Extension |
PCR/SOPH | PCR/Sequence Specific Oligonucleotide Probe Hybridization | Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization |
PCR/TMA | PCR/TMA | Polymerase Chain Reaction (PCR) and Transcription Mediated Amplification (TMA) |
PCRHPLCS | PCR/HPLC/Sequencing | Polymerase Chain Reaction/ High Performance Liquid Chromatography/Sequencing |
PETINIA | Enhanced Turbidimetric-Inhibition Immunoassay (PETINIA) | Enhanced Turbidimetric-Inhibition Immunoassay (PETINIA) |
PFA | Platelet Function Testing | (PFA)-Platelet function is measured under high shear condition which simulate those formed at sites of vascular injury. Using both an epinephrine and ADP collagen membrane, platelets undergo activation, adhesion and aggregation with progressive closure of the membrane aperture reported as the closure time in seconds. |
Plt Agg | PLATELET AGGREGATION | PLATELET AGGREGATION |
PM | Potentiometric | Potentiometric |
PM-Complex | Phosphomolybdate Complex | Phosphomolybdate Complex |
PRECIP | Precipitation | Precipitation |
Prompt | Prompt | Prompt |
Protease Z | Protease Zymogen-Based Colorimetric Assay | Protease Zymogen-Based Colorimetric Assay |
Pyro-Red | Pyrogallol Red | Pyrogallol Red |
Abbreviation | Name | Description |
Q EIA/HPLC | Qualitative EIA/Quantitative HPLC/Tandem MS | Qualitative Enzyme-Linked Immunosorbent Assay/Quantitative High Performance Liquid Chromatography/Tandem Mass Spectrometry |
Q-EIA | Quantitative Enzyme Immunoassay | Quantitative Enzyme Immunoassay |
Q-ELISA | Quantitative Enzyme-Linked Immunosorbent Assay | Quantitative Enzyme-Linked Immunosorbent Assay |
Q-EMIA | Quantitative Enzyme Multiplied Immunoassay Technique | Quantitative Enzyme Multiplied Immunoassay Technique |
Q-Enz | Quantitative Enzymatic | Quantitative Enzymatic |
Q-EQD/HPLC | Quantitative Equilibrium Dialysis/HPLC-Tandem Mass Spec. | Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry |
Q-Flow | Quantitative Flow Cytometry | Quantitative Flow Cytometry |
Q-Fluro | Quantitative Fluorometry | Quantitative Fluorometry |
Q-RIA | Quantitative Radioimmunoassay | Quantitative Radioimmunoassay |
Q-RID | Quantitative Radial Immunodiffusion | Quantitative Radial Immunodiffusion |
Q-TMA | Qualitative Transcription-Mediated Amplification | Qualitative Transcription-Mediated Amplification |
Q/SRA | Qualitative Serotonin Release Assay | Qualitative Serotonin Release Assay |
QC/EIA | Quantitative Colorimetry/Enzyme Immunoassay | Quantitative Colorimetry/Enzyme Immunoassay |
QCIA | Quantitative Chemiluminescent Immunoassay | Quantitative Chemiluminescent Immunoassay |
QE-CIA | Quantitative Electrochemiluminescent Immunoassay | Quantitative Electrochemiluminescent Immunoassay |
QEF | Quantitative Enzymatic/Fluorometry | Quantitative Enzymatic/Fluorometry |
QENZ/ELECT | Quantitative Enzymatic/Electrophoresis | Quantitative Enzymatic/Electrophoresis |
QEQ/HPLC/T | Quant Equilibrium Dialysis/HPLC-Tandem Mass Spectrometry | Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry |
QFEIA | Quantitative Fluorescent Enzyme Immunoassay | Quantitative Fluorescent Enzyme Immunoassay |
QGC/C | Quantitative Gas Chromatography/Colorimetry | Quantitative Gas Chromatography/Colorimetry |
QGC/TMS | Quantitative Gas Chromatography/Tandem Mass Spectrometry | Quantitative Gas Chromatography/Tandem Mass Spectrometry |
QGCMS | Quantitative Gas Chromatography-Mass Spectrometry | Quantitative Gas Chromatography-Mass Spectrometry |
QHI/Enz | Quantitative Heat Inactivation/Enzymatic | Quantitative Heat Inactivation/Enzymatic |
QHPLC/TMS | Quantitative HPLC/Tandem Mass Spectrometry | Quantitative High Performance Liquid Chromatography/Tandem Mass Spectrometry |
QIB/IFA | Semi-Quantitative Immunoblot/Semi-Quantitative Indirect Fluorescent Antibody | Semi-Quantitative Immunoblot/Semi-Quantitative Indirect Fluorescent Antibody |
QICP/MS | Quantitative Inductively Coupled Plasma-Mass Spectrometry | Quantitative Inductively Coupled Plasma-Mass Spectrometry |
QIFA/QELSA | Semi-Quant IFA/Semi-Quant ELISA | Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Enzyme-Linked Immunosorbent Assay |
QIT | Quantitative Immunoturbidimetric | Quantitative Immunoturbidimetric |
Ql Colo | Quantitative Colorimetry | Quantitative Colorimetry |
Ql LC/TMS | Qualitative Liquid Chromatography-Tandem Mass Spectrometry | Qualitative Liquid Chromatography-Tandem Mass Spectrometry |
QL-EIA | Qualitative Enzyme Immunoassay | Qualitative Enzyme Immunoassay |
Ql-El/RID | Qualitative Gel Electrophoresis/Radial Immunodiffusion | Qualitative Gel Electrophoresis/Radial Immunodiffusion |
Ql-ELISA | Qualitative Enzyme-Linked Immunosorbent Assay | Qualitative Enzyme-Linked Immunosorbent Assay |
QL-IB | Qualitative Immunoblot | Qualitative Immunoblot |
Ql-ID | Qualitative Immunodiffusion | Qualitative Immunodiffusion |
QL-IP/SQT- | Qualitative Immunoprecipitation/Semi-Quant Multiplex Bead Assay | Qualitative Immunoprecipitation/Semi-Quant Multiplex Bead Assay |
QL-PCR | Qualitative PCR | Qualitative Polymerase Chain Reaction |
QLC/TMS | Quant Liquid Chromatography-Tandem Mass Spectrometry | Quantitative Liquid Chromatography-Tandem Mass Spectrometry |
QlMic/St | Qualitative Microscopy/Stain | Qualitative Microscopy/Stain |
QM-BA | Quantitative Multiplexed Bead Assay | Quantitative Multiplexed Bead Assay |
QMBA | Quantitative Multiplex Bead Assay | Quantitative Multiplex Bead Assay |
Qnt-HPLC | Quantitative High Performance Liquid Chromatography | Quantitative High Performance Liquid Chromatography |
Qnt-I Chr | Quantitative Ion Chromatography | Quantitative Ion Chromatography |
Qnt-LC/Imm | Quantitative Liquid Chromatography/Immunoassay | Quantitative Liquid Chromatography/Immunoassay |
Qnt-LC/TMS | Quantitative Liquid Chromatography/Tandem Mass Spectrometry | Quantitative Liquid Chromatography/Tandem Mass Spectrometry |
QP-PCR | Repeat-Primed PCR (QP-PCR) | Repeat-Primed PCR (QP-PCR) |
QSPEC | Quantitative Spectrophotometry | Quantitative Spectrophotometry |
Qt IFA | Quantitative Indirect Fluorescent Antibody | Quantitative Indirect Fluorescent Antibody |
Qt ISE | Quantitative ion-selective electrode | Quantitative ion-selective electrode |
Qt IVD | Quantitative IVD Assay | Quantitative IVD Assay |
Qt SwEIA | Quantitative Sandwich Enzyme Immunoassay (EIA) | Quantitative Sandwich Enzyme Immunoassay (EIA) |
Qt-PCR | Quantitative Polymerase Chain Reaction | Quantitative Polymerase Chain Reaction |
Qt/ICAPFEI | Quantitative ImmunoCAP Fluorescent Enzyme Immunoassay | Quantitative ImmunoCAP Fluorescent Enzyme Immunoassay |
QU/QCIA | Quantitative Ultrafiltration/Quantitative Chemiluminescent Immunoassay | Quantitative Ultrafiltration/Quantitative Chemiluminescent Immunoassay |
Qual ELISA | Qualitative ELISA | |
Qual/If-El | Qualitative Immunofixation Electrophoresis | Qualitative Immunofixation Electrophoresis |
Quant-Neph | Quantitative Nephelometry | Quantitative Nephelometry |
Abbreviation | Name | Description |
Rapid IA | Rapid Immunoassay | Rapid Immunoassay |
Rapid-ICG | Rapid Immunochromatographic | Rapid Immunochromatographic |
RBA | Radiobinding Assay | Radiobinding Assay |
REA | Radioenzymatic Assay | Radioenzymatic Assay |
Reflect | Reflectance | Reflectance |
RFFIT | Rapid Fluorescent FOCI Inhibition Test | Rapid Fluorescent FOCI Inhibition Test |
RFIA | Rapid Fluorescent Immunoassay | Rapid Fluorescent Immunoassay |
RIA | Radioimmunoassay | (RIA) uses fixed-dose, low-level, radioactive-isotope- labeled antigen ("tracer") to compete with unlabeled antigen from the patient specimen for a fixed number of antibody binding sites. Traditional RIA is done with specific antibodies in liquid solution. Solid-phase RIA involves the use of antibody bound to solid support (e.g., tubes, glass beads or plastic fins). The amount of antigen in the specimen is determined by comparing the bound radioactivity with a standard curve. |
RIA-CHR | Radioimmunoassay/Chromatography | Radioimmunoassay/Chromatography |
RIBA/SIA | RIBA 3.0 Strip Immunoblot Assay (SIA) | RIBA 3.0 Strip Immunoblot Assay (SIA) |
RID | Radial Immunodiffusion | (RID) is a quantitative variation of the Ouchterlony technique (immunodiffusion) in which the agar gel contains evenly distributed antigen (or antibody) and its counterpart from the test sample diffuses into the gel from a single well resulting in a circular precipitin line around the sample well. The diameter of the precipitin ring is proportional to the concentration of the antibody (or antigen) present in the test sample. By comparing the diameter of the test specimen precipitin ring to known standards, a relatively insensitive estimation of the concentration of specific antibody or antigen can be achieved. |
RIPA | Radioimmunoprecipitation assay | (RIPA) is the term used to describe the qualitative assay used as a confirmatory procedure for some antibodies to viral antigens. Viral-infected cell cultures are radioactively labeled and lysed to yield radiolabeled antigen fragments. Specific antibodies, if present, will bind these antigen fragments and the resulting antigen-antibody complexes are precipitated using protein A, boiled to free the immune complexes which are then separated by electrophoresis. The pattern of antigenic moieties to which antibodies are present may then be detected using autoradiography (the exposure of sensitive X-ray film by the radioactive emissions of the bound, labeled antigens). Comparison to labeled molecular weight standards electrophoresed in the same run allows determination of the molecular weight "bands" of antigen to which antibodies are present. |
RIPA | RIPA gel radiography | RIPA gel radiography |
RT-PCR | Reverse Transcriptase PCR | (RT-PCR) is a technique used to amplify RNA targets. The specimen containing the target RNA (e.g., HIV-1 RNA, Hepatitis C Virus RNA) is subjected to reverse transcription to make complementary DNA (cDNA), which is then, in turn, amplified by PCR. |
Abbreviation | Name | Description |
S-Q IFA | Semi-Quantitative Indirect Fluorescent Antibody | Semi-Quantitative Indirect Fluorescent Antibody |
SB | Southern Blot | (SB) describes the technique first developed by the Scottish molecular biologist Edward M. Southern which now bears his name. Specimen DNA is denatured, treated with restriction enzymes to result in DNA fragments and then the single-stranded DNA fragments are separated by electrophoresis. The electrophoretically separated fragments are then blotted to a nitrocellulose membrane, retaining their electrophoretic position, and hybridized with radiolabeled single- stranded DNA fragments with sequences complementary to those being sought. The resulting double-stranded DNA bearing the radiolabel is then, if present, detected by autoradiography. |
SDA | Strand displacement amplification | Strand displacement amplification |
See Notes | See Test Notes | See Test Notes |
SEMI-FC | Semi-Quantitative Flow Cytometry | Semi-Quantitative Flow Cytometry |
Semi-Q RID | Semi-Quantitative Radial Immunodiffusion | Semi-Quantitative Radial Immunodiffusion |
SF | Scanning Fluorometry | Scanning Fluorometry |
SNP MA | SNP Microarray Analysis | SNP microarray analysis |
Solubility | Solubility | Solubility |
SPEA | Spectrophotometric, enzymatic assay | Spectrophotometric, enzymatic assay |
SPEC | Spectrophotometry | Spectrophotometry |
SpecInfo | Specimen Information | Specimen Information |
SPI | SPI | Solid-Phase Immunochromatograph |
SQ-EIA | Semi-Quantitative Enzyme Immunoassay | Semi-Quantitative Enzyme Immunoassay |
SQ-ELISA | Semi-Quantitative Enzyme-Linked Immunosorbent Assay | Semi-Quantitative Enzyme-Linked Immunosorbent Assay |
SQ-IFA | Semi-Quantitative Immunofluorescence Assay | Semi-Quantitative Immunofluorescence Assay (Indirect Fluorescent Antibody) |
SQ-SN | Semi-Quantitative Serum Neutralization | Semi-Quantitative Serum Neutralization |
SQAGG | Semi-Quantitative Agglutination | Semi-Quantitative Agglutination |
SQBio/QCIA | Semi-Quant Bioassay/Quant Chemiluminescent Immunoassay | Semi-Quant Bioassay/Quant Chemiluminescent Immunoassay |
SQCF | Semi-Quantitative Complement Fixation | Semi-Quantitative Complement Fixation |
SQt-RIA | Semi-Quantitative Radioimmunoassay | Semi-Quantitative Radioimmunoassay |
SSI | Single Stain Immunofluorescence | Single Stain Immunofluorescence |
SV | Shell Vial Culture | Shell Vial Culture |