| Nt | Neutralization | (Nt) is similar to complement fixation but is applicable only in certain pathogenic situations where the antibody being measured is directed against a hemolysin (a bacterial toxin capable of directly lysing erythrocytes). In these situations, the hemolysin and reagent erythrocytes are added, and if the antibody to the hemolysin is present, the lysis of RBCs will not occur. As in CF, crude quantitation is afforded by serial dilution which may be quantitatively compared to established standard material dilutions. |