Paroxysmal Nocturnal Hemoglobinuria (PNH)(CD55/CD59)
Order Name
CD55/CD59
Test Number: 0123875
Revision Date 04/27/2009
Test Number: 0123875
Revision Date 04/27/2009
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
Percent Normal RBCs
|
Flow cytometry | |
|
Percentage PNH Cells
|
Flow cytometry |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | 5 mL (0.2) | Whole Blood | EDTA (Lavender Top) | Room Temperature |
| Alternate 1 | 5 mL (0.2) | Whole Blood | Sodium Heparin (Green Top / No-Gel) | Room Temperature |
| Instructions | Stability: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable. | |||
| GENERAL INFORMATION | |
|---|---|
| Testing Schedule | Sun-Sat |
| Expected TAT | 2-4 Days |
| Clinical Use | The High Resolution PNH-RBC panel detects the absence of phosphatidylinositol-linked membrane proteins CD55 (DAF-decay accelerating factor) and CD59 (MIRL-membrane inhibitor of reactive lysis) on erythrocytes. The erythrocytes are identified with glycophorin staining. All normal RBCs (99.995-100.000%) express CD55 and CD59 and are reported as % Normal RBCs. The expression of CD55 and CD59 is decreased or absent in a subpopulation of RBCs in patients with paroxysmal nocturnal hemoglobinuria. The estimated percentage of RBCs with decreased expression is reported as % PNH cells. |
| CPT Code(s) | 88184, 88185 |
| Internal Comments | OLD NOTES (PRE JAN 2008)- The expression of CD55 and CD59 is decreased or absent in patients with paroxysmal nocturnal hemoglobinuria (PNH). All normal RBCs (100%) express CD55 and CD59. The estimated percentage of RBCs with decreased expression is reported as percent PNH cells. |
| Lab Section | Reference Lab |