Vitamin D, 25-Hydroxy Total (Vit D 25-OH)
Order Name
VITAMIN D
Test Number: 3604940
Revision Date 07/20/2009
Test Number: 3604940
Revision Date 07/20/2009
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
Vitamin D, 25-Hydroxy Total (Vit D 25-OH)
|
Chemiluminescence Assay |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | 1 mL (0.3) | Serum | Clot Activator SST | Frozen |
| Alternate 1 | 1 mL (0.3) | Plasma | EDTA (Lavender Top) | Frozen |
| Instructions | Stability: After separation from cells: Ambient: 3 days; Refrigerated: 7 days; Frozen: 6 months. *(Note: If ordering both Vitamin D 25OH & Vitamin D 1-25OH then seperate serum into two seperate aliquots and freeze. See collection instructions for Vitamin D 1-25 Dihydroxy.) EDTA and Heparin Plasma is also acceptable. |
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| GENERAL INFORMATION | |
|---|---|
| Testing Schedule | Sun-Sat |
| Expected TAT | 2-3 Days |
| Clinical Use | This assay accurately quantifies the sum of Vitamin D3 25-Hydroxy (the endogenous form) and Vitamin D2 25-Hydroxy (the supplement form). Optimum 25-hydroxy Vitamin D concentration is 35 ng/mL or higher. |
| Notes | Reports total Vitamin D, individual Vitamin D2 and D3 values will not be resulted as of October 16th, 2007. |
| CPT Code(s) | 82306 |
| Internal Comments | old clinical data:Measurement of Vitamin D 25-OH (D2) is an indicator of compliance with supplements. Levels greater than 4 ng/mL suggest compliance. Vitamin D 25-OH (D3) indicates endogenous production. Therapy should be based on measurement of total Vitamin D 25-OH, with levels less than 20 ng/mL suggesting Vitamin D deficiency and levels between 20 ng/mL and 30 ng/mL suggesting a possible need for supplementation. |
| Lab Section | Reference Lab |