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Monospot Test (Mono Test)

Order Name MONO TEST
Test Number: 5504950
Revision Date 09/10/2021
Test Name Methodology LOINC Code
Monospot Test (Mono Test)
Immunoassay 31418-7 
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 1 mL Serum Clot Activator SST Refrigerated
Alternate 1 1 mL Plasma EDTA (Lavender Top) Refrigerated
Alternate 2 1 mL Plasma Lithium Heparin PST (Light Green Top) Refrigerated
Instructions Serum should be allowed to clot at room temperature, then centrifuged at 1500 x g for 10 minutes. Serum should be separated ASAP.
Refrigerate all specimens at 2°-8°C until ready for testing. If serum or plasma specimens will not be tested within 48hrs, they should be stored at or below -20°C if not tested within 48hrs.
Reject criteria: Grossly hemolyzed specimens.

Note: Further diagnostic workup and/or serology testing is available upon request. Serum is the only acceptable specimen for these serology tests.
Testing Schedule Daily 
Expected TAT 1 Day  
Clinical Use Diagnosis of Mononucleosis. Suggest performing an EBV panel if the monospot test is negative. 
CPT Code(s) 86308
Lab Section Hematology